کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534493 1551171 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of immuno-PCR assay for the detection of serum IgE specific to Bermuda allergen
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Application of immuno-PCR assay for the detection of serum IgE specific to Bermuda allergen
چکیده انگلیسی


- A sensitive iPCR for serum IgE specific to Bermuda allergen is presented.
- The assay demonstrates high specificity and sensitivity for serum IgE.
- This iPCR is 100-fold sensitive for serum IgE compared to conventional ELISA kit.

In vivo and in vitro tests are the two major ways of identifying the triggering allergens in sensitized individuals with allergic symptoms. Both methods are equally significant in terms of sensitivity and specificity. However, in certain circumstances, in vitro methods are highly preferred because they circumvent the use of sensitizing drugs in patients. In current study, we described a highly sensitive immuno-PCR (iPCR) assay for serum IgE specific to Bermuda allergens. Using oligonucleotide-labelled antibody, we used iPCR for the sensitive detection of serum IgE. The nucleotide sequence was amplified using conventional PCR and the bands were visualized on 2.5% agarose gel. Results demonstrated a 100-fold enhancement in sensitivity of iPCR over commercially available enzyme-linked immunosorbent assay (ELISA) kit. Our iPCR method was highly sensitive for Bermuda-specific serum IgE and could be beneficial in allergy clinics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 1-4
نویسندگان
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