کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534517 1402183 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening and identification of a specific peptide for targeting hypoxic hepatoma cells
ترجمه فارسی عنوان
غربالگری و شناسایی یک پپتید خاص برای هدف گیری سلول های هپاتوما هیپوکسیک
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


- A heptapeptide that bind specifically to hypoxic hepatoma cells is reported.
- We use a phage display peptide library for in vivo and in vitro screening to obtain this pepetide.
- The binding assay indicated that the heptapeptide could specifically bind to hypoxic hepatoma cells and hepatocarcinoma tissues.
- The peptide has the potential to be a useful targeting ligand for residual hepatoma cells after TACE.

The biological behaviors of residual hepatoma cells after transarterial embolization therapy, which exist in a hypoxic or even anaerobic tumor microenvironment, differ from the tumor cells under normoxic conditions. This study aimed to use a phage display peptide library for in vivo and in vitro screening to obtain a peptide which could specifically bind to hypoxic hepatoma cells, allowing further targeted diagnosis and treatment for liver cancer. In this study, hypoxic hepatoma cells HepG2 (targeted cells), and normal liver cells HL-7702 (control cells), were utilized to perform three rounds of in vitro screening using a phage-displayed 7-mer peptide library. In addition, hypoxic HepG2 were subcutaneously injected into nude mice to establish a hepatocarcinoma model, followed by performing three rounds of in vivo screening on the phages identified from the in vitro screening. The products from the screening were further identified using ELISA and immunofluorescence staining on cells and tissues. The results indicated that the P11 positive clone had the highest binding effect with hypoxic hepatoma cells. The sequence of the exogenous insert fragment of P11 positive clone was obtained by sequencing: GSTSFSK. The binding assay indicated that GSTSFSK could specifically bind to hypoxic hepatoma cells and hepatocarcinoma tissues. This 7-mer peptide has the potential to be developed as an useful molecular to the targeting diagnosis and treatment of residual hepatoma cells after transarterial chemoembolization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 30, Issue 4, August 2016, Pages 246-253
نویسندگان
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