کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534551 1551172 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle
چکیده انگلیسی


- A LAMP assay for specific detection of Theileria annulata in cattle was evaluated in a field trial.
- A detection of a 0.00025% parasitaemia was achieved.
- The assay is useful for simple and rapid detection of T. annulata from clinical samples.

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 31, February 2017, Pages 61-64
نویسندگان
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