|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5534552||1402185||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
- We report an improved real-time PCR for quantifying Leishmania spp. in canine blood samples.
- The assay detects less than a single parasite cell per reaction.
- The assay uses a small final reaction volume (15Â Î¼l), reducing costs.
- The assay takes less than 34Â min to perform.
We describe an improved real-time PCR assay (designated as “Leishmania-FAST15”) for the detection and quantification of Leishmania infantum and Leishmania braziliensis kinetoplast DNA minicircles in canine blood samples. The analytical sensitivity of this technique is 0.1Â fg of DNA, which is equivalent to 0.002 parasite per reaction. This assay uses a small reaction volume (15Â Î¼l) and is rapid to perform, with the results being available in less than 34Â min. This improved assay might also be suitable for detecting and quantifying L.Â infantum and L.Â braziliensis DNA in other tissues, such as bone marrow and lymph nodes.
Journal: Molecular and Cellular Probes - Volume 31, February 2017, Pages 65-69