Short communicationRapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay

- A rapid and sensitive AHPND-RPA assay was developed for the detection of the AHPND-causing agent of Vibrio owensii.
- The AHPND-RPA assay detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C.
- The AHPND-RPA assay showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.