کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5535153 | 1402217 | 2017 | 6 صفحه PDF | دانلود رایگان |
- 3D culture plays an important role in differentiation of hUCMs into IPC.
- Gene expression was increased in the fibrin scaffold culture.
- Insulin and pro-insulin protein were amplified in fibrin scaffold culture.
- Insulin secretion was increased in the fibrin scaffold culture.
- Differentiation in fibrin scaffold was responded to glucose challenge test more than conventional culture method.
Tissue engineering is a new strategy which proposed to treat numerous human diseases nowadays. Three dimensional (3D) scaffolds fill the gap between two dimensional cell culture (2D) and animal tissues through mimicking the environmental behaviors surrounding the cells. In this study, hUCMs into insulin producing cells in fibrin scaffold were differentiated compare to conventional culture condition. Differentiation rate was estimated by real time PCR, immunocytochemistry (ICC) and the chemiluminesence (CLIA) and enzyme immunoassay (EIA). Real time PCR's results showed an increasing expression in NKX2.2, PDX1 and INS (producing the hormone insulin) genes in fibrin scaffold. Furthermore ICC analysis exhibited that insulin and pro-insulin proteins were more in fibrin scaffolds. CLIA and EIA on insulin and C peptide secretion indicated that both of groups were sensitive to the glucose challenge test but significant higher response was observed in fibrin scaffold (6.5 fold in 3D, 1.8 fold in 2D culture). It could be concluded that differentiation of hUCM cells into insulin producing cells in fibrin scaffold 3D culture system is much more efficient than 2D conventional culture system.
Journal: Tissue and Cell - Volume 49, Issue 2, Part B, April 2017, Pages 227-232