کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5543416 1554069 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues
ترجمه فارسی عنوان
توسعه یک آنزیم ایمونوسیورسانس متشکل از آنزیم شناسایی شده برای تشخیص کلیندامایسین و بقایای لینکومایسین در بافت های خوراکی حیوانی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی
In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra −/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC − MS/MS with a correlation coefficient > 0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 125, March 2017, Pages 137-142
نویسندگان
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