In vitro susceptibility of Brachyspira hyodysenteriae to a commercial citrus fruit extract

The results showed that BIOCITRO has a relevant bacteriostatic and bactericidal effect against B. hyodysenteriae with MIC and MBC values ranging from 32 to 128 parts per million (ppm). It induces damage in at least 35% and 76% of the bacterial cells when exposed to 128 and 256 ppm of BIOCITRO respectively as revealed by the intake of propidium iodide by FC. Relevant changes in the structure of the bacterial cells were observed by SEM and confirmed by FTIR. According to these results, BIOCITRO seems to be a satisfactory alternative to the use of antibiotics in the control of SD.