کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5543841 | 1554296 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative pathogenicity and immunogenicity of triple and double gene-deletion pseudorabies virus vaccine candidates
ترجمه فارسی عنوان
پاتوژنز مقایسه و ایمنی زایی نامزدهای واکسن ویروس سه گانه و دو ژن حذف
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Pseudorabies (PR) outbreaks have re-emerged in many pig farms with vaccination coverage in China, which suggests that current vaccine strains provide poor protection against novel, epidemic strains. In this study, based on the highly virulent PRV ZJ01 variant, a PR virus (PRV) thymidine kinase (TK)/gE/gI deleted strain-rZJ01ÎTK/gE/gI-was generated, which showed similar growth features in vitro compared to the parent strain PRV ZJ01 and its gE/gI deleted strain rZJ01ÎgE/gI. The results of a piglet experiment (with 10 piglets each group) showed that the rZJ01ÎTK/gE/gI vaccine generated similar levels of neutralizing antibodies against ZJ01 compared to the rZJ01ÎgE/gI vaccine (p > 0.05). However, rZJ01ÎgE/gI inoculation resulted in slight inflammatory cell infiltrations, hemorrhages, and congestion in the brain and lungs. After a ZJ01 challenge, all animals in the rZJ01ÎTK/gE/gI- and rZJ01ÎgE/gI-vaccinated groups survived without exhibiting any clinical symptoms, whereas all non-vaccinated control animals died within 7 days post-challenge. Furthermore, microscopic lesions and virus loads in the brains and lungs in the two vaccinated groups were significantly lower than those in the control group. Meanwhile, the virus levels in the brains of piglets in the rZJ01ÎTK/gE/gI group were significantly lower than those in the rZJ01ÎgE/gI group. These results indicate that the triple gene-deleted PRV rZJ01ÎTK/gE/gI strain has lower pathogenicity and higher protective efficacy against variant PRV challenge compared with the double gene-deleted PRV rZJ01ÎgE/gI strain. Together, all these data indicate that the PRV rZJ01ÎTK/gE/gI strain should be an ideal vaccine candidate for the prevention of PR in China.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 115, December 2017, Pages 17-23
Journal: Research in Veterinary Science - Volume 115, December 2017, Pages 17-23
نویسندگان
Jing Dong, Juan Bai, Tao Sun, Zhenqing Gu, Jichun Wang, Haifeng Sun, Ping Jiang,