کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5545877 | 1555642 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Anti-helminthic activity of the essential oil of Lippia sidoides against monogeneans of Colossoma macropomum is dose-dependent.
- Lippia sidoides killed the parasites after 10Â min in the in vitro trial.
- Parasites exposed to water died after 8Â h in the in vitro immobilization test.
- Lippia sidoides causes anesthetic effect in fish and gills histopathological alterations.
In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320Â mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160Â mg/L were 100% effective against monogenoideans in 10Â min and 1Â h of exposure, respectively. However, the effectiveness of 100% concentrations of 80Â mg/L and 40Â mg/L occurred in 3 and 6Â h, respectively. In the in vivo tests, juvenile fish were submitted to 60Â min of baths with 10Â mg/L and 15Â min of baths with 20Â mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20Â mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24Â h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites.
Journal: Veterinary Parasitology - Volume 234, 30 January 2017, Pages 49-56