کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5550091 1557283 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Delivery of cyclodextrin polymers to bacterial biofilms - An exploratory study using rhodamine labelled cyclodextrins and multiphoton microscopy
ترجمه فارسی عنوان
تحویل پلیمرهای سیکلوکود کسترین به بیوفیلم های باکتری - یک مطالعه اکتشافی با استفاده از سیکلوکودکسترین های برچسب ردمامین و میکروسکوپ چند فتونی
کلمات کلیدی
رودامین، پلیمرهای سیکلدکسترین، کاربردکردن شارژ، بیوفیلم استافیلوکوک اپیدرمیدیس، میکروسکوپ اسکن لیزر چند فتونی، تجزیه طیفی،
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
چکیده انگلیسی

Cyclodextrin (CD) polymers are interesting nanoparticulate systems for pharmaceutical delivery; however, knowledge regarding their applications towards delivery into complex microbial biofilm structures is so far limited. The challenge is to demonstrate penetration and transport through the biofilm and its exopolysaccharide matrix. The ideal functionalization for penetration into mature biofilms is unexplored. In this paper, we present a novel set of rhodamine labelled βCD-polymers, with different charge moieties, i.e., neutral, anionic, and cationic, and explore their potential delivery into mature Staphylococcus epidermidis biofilms using multiphoton laser scanning microscopy (MPM). The S. epidermidis biofilms, being a medically relevant model organism, were stained with SYTO9. By using MPM, three-dimensional imaging and spectral investigation of the distribution of the βCD-polymers could be obtained. It was found that the cationic βCD-polymers showed significantly higher integration into the biofilms, compared to neutral and anionic functionalized βCDs. None of the carriers presented any inherent toxicity to the biofilms, meaning that the addition of rhodamine moiety does not affect the inertness of the delivery system. Taken together, this study demonstrates a novel approach by which delivery of fluorescently labelled CD nanoparticles to bacterial biofilms can be explored using MPM. Future studies should be undertaken investigating the potential in using cationic functionalization of CD based delivery systems for targeting anti-microbial effects in biofilms.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 531, Issue 2, 15 October 2017, Pages 650-657
نویسندگان
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