کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5551861 1557805 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice
چکیده انگلیسی


- Hantavirus (HTV) DNA vaccine pVAX-LAMP/Gn established memory immune responses.
- Only pVAX-LAMP/Gn gained long-term seroconversion compared with inactivated vaccine.
- Enhanced CTL cytotoxicity and Th IFN-γ activity defined cellular memory immunity.
- Epitope-spreading phenomenon suggested cross-species T-cell hot-spot on Hantaan virus Gn.
- Safety of pVAX-LAMP/Gn was reconfirmed through behavioral and histological analysis.

BackgroundProphylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring long-term evaluations.Methods & resultsImmune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species.ConclusionpVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 138, February 2017, Pages 32-39
نویسندگان
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