کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5553273 | 1557953 | 2017 | 9 صفحه PDF | دانلود رایگان |
BackgroundProstate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties.MethodsWe investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts (“biological”- in vitro digested, “physical”- size exclusion, and “chemical”-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin.ResultsTime course analysis (by 24Â h, 48Â h and 72Â h) of LJP (1-0.1Â mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03-0.003Â mg/mL) after 72Â h exposure showed potent growth inhibitory (IC50Â =Â 0.02-0.07Â mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells.ConclusionThis is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.
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Journal: Biomedicine & Pharmacotherapy - Volume 89, May 2017, Pages 515-523