کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5556557 1560479 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid assessment for predicting drug-induced hepatotoxicity using zebrafish
ترجمه فارسی عنوان
ارزیابی سریع برای پیشگیری از سمیت کبد ناشی از دارو با استفاده از زبرا ماهی
کلمات کلیدی
ماهی قزل آلا، سمیت کبدی، شاخص سطح کبد، ارزیابی هپاتوتوکسیک، مدل حیوانی، مواد و روش ها،
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
چکیده انگلیسی

IntroductionZebrafish have been used as a model to access drug-induced hepatotoxicity. However, individual differences occur in the liver development of zebrafish.MethodsWe used a transgenic line of zebrafish that expressed enhanced green fluorescent protein (EGFP) in the liver and then used a calculation of the liver index area, a potentially new endpoint of hepatotoxicity, to evaluate drug-induced liver injury. To further validate the reliability of the liver area index as a quick evaluation of zebrafish liver function damage, the liver area index level was correlated with hepatic transaminase activities using the Pearson correlation coefficient and confirmed by histopathology.ResultsZebrafish larvae treated with high doses of the known mammalian hepatotoxic drugs carbaryl, isoniazide, and pyrazinamide showed significantly decreased liver area index levels, which are suggestive of liver injury and correspond with the higher alanine transaminase (ALT) and aspartate transaminase (AST) activities and histological liver alterations. The results showed a significant negative correlation between the degree of liver injury and the liver area index level.DiscussionOur data support the use of the liver area index as a reliable and comparable indicator to screen hepatotoxic agents using the zebrafish model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacological and Toxicological Methods - Volume 84, March–April 2017, Pages 102-110
نویسندگان
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