کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5556863 | 1560546 | 2017 | 7 صفحه PDF | دانلود رایگان |
AimsWe examined the roles of xanthine oxidoreductase (XOR) in renal ischemia reperfusion (IR) injury.Main methodsXOR +/+ and XOR +/â mice were subjected to 24-h reperfusion after a 45-min bilateral renal artery occlusion or sham operation. We evaluated the renal damage based on the concentrations of blood urea nitrogen (BUN) and serum creatinine (Cr), and histological changes were detected by PAS staining. Xanthine dehydrogenase, oxidase (XO) and XOR activities, amounts of blood and urine 8-OHdG, and expressions of TNF-α and MCP-1 mRNA were examined. F4/80 and nitrotyrosine-positive cells were assessed by immunohistochemical staining.Key findingsThe BUN and Cr concentrations in the XOR +/+IR mice were increased significantly compared to those in XOR +/âIR and allopurinol-treated XOR +/+IR mice. XO and XOR activity, which were increased in IR mice, were reduced in the allopurinol-treated XOR +/+IR and XOR +/âIR mice compared to the XOR +/+IR mice. The concentrations of blood and urine 8-OHdG, and the expressions of MCP-1 and TNF-α mRNA were increased significantly in the XOR +/+IR mice compared to those in the XOR +/âIR mice. The histological analysis revealed that the XOR +/âIR and allopurinol-treated XOR +/+IR mice showed less tubular injury than the XOR +/+IR mice in the cortex regions, with the reduction of inflammation and oxidative stress assessed by the immunohistological staining for F4/80 and nitrotyrosine.SignificanceBoth the disruption of XOR gene in XOR +/â mice and the reduction of XOR activity in allopurinol-treated XOR +/+IR mice attenuated renal tissue injury in this IR model. Reduced XOR activity during renal IR could be a beneficial treatment target.
Journal: Life Sciences - Volume 182, 1 August 2017, Pages 73-79