کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5556914 1560549 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nerve growth factor-carbon nanotube complex exerts prolonged protective effects in an in vitro model of ischemic stroke
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Nerve growth factor-carbon nanotube complex exerts prolonged protective effects in an in vitro model of ischemic stroke
چکیده انگلیسی

AimsThe therapeutic potential of nerve growth factor (NGF) against the neurological disorders may be negatively affected by its short half-life. Based on the superior properties of carbon nanotubes (CNTs) for controlled drug delivery, we aimed to prepare CNT-NGF complex and evaluate its effect in an in vitro model of ischemic stroke.Materials and methodsMulti-walled CNTs (MWCNTs)-NGF complex was prepared using amino-functionalized COOH-MWCNTs and characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. PC12 cells in the absence or presence of NGF (0.5, 1, 2 μg/ml), acid- or amine-modified MWCNTs, or MWCNTs-NGF complex (2, 4, 8 μg/ml) were exposed to 1 and 6 h oxygen-glucose deprivation (OGD) followed by 24 h re-oxygenation. Cytotoxicity and oxidative stress were evaluated.Key findingsOGD significantly reduced the cell viability (P < 0.001). NGF dose-dependently increased the cell viability only after 1-h OGD (P < 0.05), while, MWCNTs-NGF complex was effective at both 1- and 6-h OGD (P < 0.05, P < 0.001). NGF reduced the formation of condensed nuclei due to 1-h OGD (P < 0.01, P < 0.001), while, MWCNTs-NGF showed efficiency at both time points (P < 0.05, P < 0.01, P < 0.001). OGD significantly increased malondialdehyde (MDA) content and decreased catalase (CAT) and superoxide dismutase (SOD) activities (P < 0.001). After 1-h OGD, NGF reduced MDA (P < 0.001) and increased CAT (P < 0.05, P < 0.01) and SOD (P < 0.01), while, MWCNTs-NGF was effective after both 1- and 6-h OGD (MDA: P < 0.01, P < 0.001, CAT: P < 0.001, SOD: P < 0.01, P < 0.001).SignificanceAminated MWCNTs-NGF complex by providing longer lasting effects for NGF might be of therapeutic significance against the disorders associated with NGF deficiency.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 179, 15 June 2017, Pages 15-22
نویسندگان
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