کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5557408 | 1560824 | 2017 | 8 صفحه PDF | دانلود رایگان |
The pituitary adenylate cyclase-activating polypeptide (PACAP)-27 modulates various biological processes, from the cellular level to function specification. However, the cardiac actions of this neuropeptide are still under intense studies. Using control (+|+) and mice lacking (â|â) either R-type (Cav2.3) or T-type (Cav3.2) Ca2+ channels, we investigated the effects of PACAP-27 on cardiac activity of spontaneously beating isolated perfused hearts. Superfusion of PACAP-27 (20 nM) caused a significant increase of baseline heart frequency in Cav2.3(+|+) (156.9 ± 10.8 to 239.4 ± 23.4 bpm; p < 0.01) and Cav2.3(â|â) (190.3 ± 26.4 to 270.5 ± 25.8 bpm; p < 0.05) hearts. For Cav3.2, the heart rate was significantly increased in Cav3.2(â|â) (133.1 ± 8.5 bpm to 204.6 ± 27.9 bpm; p < 0.05) compared to Cav3.2(+|+) hearts (185.7 ± 11.2 bpm to 209.3 ± 22.7 bpm). While the P wave duration and QTc interval were significantly increased in Cav2.3(+|+) and Cav2.3(â|â) hearts following PACAP-27 superfusion, there was no effect in Cav3.2(+|+) and Cav3.2(â|â) hearts. The positive chronotropic effects observed in the four study groups, as well as the effect on P wave duration and QTc interval were abolished in the presence of Ni2+ (50 μM) and PACAP-27 (20 nM) in hearts from Cav2.3(+|+) and Cav2.3(â|â) mice. In addition to suppressing PACAP's response, Ni2+ also induced conduction disturbances in investigated hearts. In conclusion, the most Ni2+-sensitive Ca2+ channels (R- and T-type) may modulate the PACAP signaling cascade during cardiac excitation in isolated mouse hearts, albeit to a lesser extent than other Ni2+-sensitive targets.
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Journal: Pharmacological Research - Volume 117, March 2017, Pages 140-147