|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5562485||1403426||2018||8 صفحه PDF||سفارش دهید||دانلود کنید|
- In stimulated theca cells, PFCs inhibit progesterone and androstenedione secretion.
- In stimulated granulose cells, PFCs inhibit progesterone and estradiol secretion.
- PFOS and PFOA act as endocrine disruptors in steroidogenic ovaric cells.
Previous studies with perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) indicate that they act as endocrine disruptors, in addition to inducing alterations and damaging reproductive health; however, the biological mechanisms by which these disorders are produced are not yet understood. The aim of this study was to analyze the effect of PFOS and PFOA on in vitro steroidogenic secretion in porcine theca and granulosa cells, with or without gonadotropic stimulation. Granulosa and theca cells were isolated and cultured. Cell nature was performed by immunocytochemistry. PFOS and PFOA effect on steroid secretion was analyzed by chemiluminescence. In the present study, alterations in steroidogenic secretion were found when administering PFOS (0.12, 1.2, 12, 120 or 240Â Î¼M) or PFOA (0.012, 0.12, 1.2, 12 or 24Â Î¼M) to theca and granulosa cells. When theca and granulosa cells were stimulated with 500Â ng/mL LH or 500Â ng/mL FHS, respectively and immediately followed with 1.2Â Î¼M of PFOS or PFOA, the perfluorinated compounds inhibited the secretion of steroid hormones in both stimulated cell types. The results indicate that PFOS and PFOA act on steroidogenic ovarian cells as endocrine disruptors, which could affect the dependent functions of sexual steroids.
Journal: Toxicology in Vitro - Volume 46, February 2018, Pages 86-93