کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562764 1562707 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology
چکیده انگلیسی


- We have established a new brain cell line (DLB-1) from European sea bass.
- DLB-1 cells show rapid growth and glial origin.
- Metals produce cytotoxicity in DLB-1 being Cd the most toxic.
- DLB-1 cell line might be useful for fish neurotoxicological studies.

In teleost fish, there are no commercial cell lines for the European sea bass (Dicentrarchus labrax). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly. Moreover, all the metals induce apoptosis as indicated by sub-Go/G1 population and annexin V binding. Finally, exposure of DLB-1 cells to metals also produces significant alterations at gene expression level, which confirm the above functional results. This is the first study in which metal cytotoxicity has been evaluated in a fish brain cell line and results seem to support that DLB-1 cells are suitable for toxicological studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 38, February 2017, Pages 91-100
نویسندگان
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