کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5586820 | 1568713 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endothelial immunocytochemical expression of pituitary IL-1β and its relation to ACTH-positive cells is regulated by corticosterone in the male rat
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کلمات کلیدی
ACTHPAPCVOIL-1R1ADXIL-1βPOMCHRPDABLPSTBSPBS3,3′-diaminobenzidine - 3،3'-diaminobenzidineECs - EC هاcircumventricular organs - اندامهای دور رحمیInterleukin-1β (IL-1β) - اینترلوکین 1β (IL-1β)Interleukin-1 beta - اینترلوکین-1 بتاEndothelial cells - سلولهای اندوتلیالPituitary - غدهٔ زیرمغزی یا هیپوفیزPhosphate buffered saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکاریدHPA - میلی بار یا هکتوپاسکالinterleukin-1 receptor type 1 - نوع 1 گیرنده اینترلوکین 1adrenocorticotropic hormone - هورمون adrenocorticotropichypothalamus-pituitary-adrenal - هیپوتالاموس-هیپوفیز-آدرنالHorseradish peroxidase - پراکسیداز هوررادیشperoxidase-antiperoxidase - پراکسیداز- آنتی پراکسیدازproopiomelanocortin - پروپیوملانوکورتینGlucocorticoids - گلوکوکورتیکوئیدهاIce - یخ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Interleukin-1 beta (IL-1β) is a cytokine linking the neuroendocrine system and metabolic homeostasis. We have previously demonstrated the relevance of IL-1β for maintaining the pituitary ACTH-producing cells by immuno-blocking its effects in pituitary cultures. However, the morphological characteristics and the intimate relationship of the pituitary cells expressing IL-1β and ACTH remain unknown. For determining pituitary variations of immunoreactivity for IL-1β and its relation with ACTH-positive cells under stress situations, we performed an immunohistochemical analysis of the expression of IL-1β and ACTH in the pituitary gland of adult rats, in the absence or presence of corticosterone, by establishing different groups: untreated, sham-operated, and bilaterally adrenalectomized animals. In the rats subjected to surgery, the glucocorticoid was administered on the same day of the intervention and on the third day post-surgery. Interestingly, it was observed that IL-1β was located in the pituitary endothelial cells at the hypophyseal portal vessels, regardless of the treatment schedule. When comparing the pituitary immunoreactive surface to IL-1β expression without corticosterone, adrenalectomized animals displayed a significantly greater area than the sham-operated animals. Corticosterone significantly inhibited the effect of adrenalectomy depending on the time interval it was administered. By in situ hybridization, IL-1β mRNA expression was also correlated with immnunocytochemical expression of pituitary IL-1β. Our results demonstrate that IL-1β is a constitutive element in endothelial portal pituitary vessels and under stress experimental conditions IL-1β increases its expression and its relation with ACTH-positive cells, suggesting that IL-1β could participate in an autocrine-paracrine fashion thereby modulating the pituitary population of ACTH-positive cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 99, November 2017, Pages 9-17
Journal: Cytokine - Volume 99, November 2017, Pages 9-17
نویسندگان
MarÃa Carmen Iglesias-Osma, Enrique J. Blanco, Marta Carretero-Hernández, MarÃa José GarcÃa-Barrado, Leonardo Catalano-Iniesta, Manuel Carretero, Virginia Sánchez-Robledo, José Carretero,