کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5586827 | 1568713 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Osteopontin in hepatocellular carcinoma: A possible biomarker for diagnosis and follow-up
ترجمه فارسی عنوان
استئوپنتین در کارسینوم سلول های بنیادی: بیومارکسیون احتمالی برای تشخیص و پیگیری
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
Recently osteopontin (OPN), a protein of the extracellular matrix, has generated in hepatocellular carcinoma (HCC) a significant interest as a prognostic factor. Aim of this study was to confirm, in liver tissues of subjects with HCV-positive HCC undergoing liver transplantation (RL, n = 10) and of donors (DL, n = 14), the increase of OPN plasma and tissue concentration, the OPN splicing isoforms expression profiling together with those of thrombin, and to evaluate a possible association between OPN measurements. Their association with Notch-1, IV-Collagen-7s domain, IL-6 and TNF-α were also evaluated. Real-Time PCR experiments and immunometric assay were performed. mRNA expression resulted higher in RL than in DL for all analyzed genes and several correlations were found between them. The more relevant association were between OPN-a and OPN-b (p < 0.0001), between thrombin and OPN-a (p = 0.007), between 7s-collagen and OPN isoforms (p < 0.05) and between Notch-1 with OPN-c (p = 0.004). Both OPN plasma and liver tissue extract concentrations were assessed confirming the trend observed at the mRNA level. An important association was found between OPN plasma and protein (p < 0.0001, r = 0.96) even splitting patients in DL (p < 0.0001, r = 0.93) and RL (p < 0.0001, r = 0.96). A reduction of OPN plasma levels was found at 6 months after transplantation. Considering MELD score as liver disease severity, the mRNA expression of our markers as well as of OPN plasma and tissue concentrations resulted increased as a function of clinical severity. Our results might be considered a useful starting point to validate OPN as a prognostic and diagnostic marker of HCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 99, November 2017, Pages 59-65
Journal: Cytokine - Volume 99, November 2017, Pages 59-65
نویسندگان
Manuela Cabiati, Melania Gaggini, Maria Michela Cesare, Chiara Caselli, Paolo De Simone, Franco Filipponi, Giuseppina Basta, Amalia Gastaldelli, Silvia Del Ry,