کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589210 | 1569807 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning, characterization, and expression of duck 2â²-5â²-oligoadenylate synthetase-like gene
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CARDScaspase activation and recruitment domainsOASSNucleotidyltransferaseOASLRIG-IUblISGs - ISG هاubiquitin-like - ubiquitin مانندDuck - اردکDuck Tembusu virus - اردک می شود ویروسinterferon - اینترفرونIFN - اینترفرون هاNeighbour-joining - همسایگیAntiviral protein - پروتئین ضد ویروسیretinoic acid-inducible gene I - ژن I القاء شده اسید رتینوئیکInterferon-stimulated genes - ژن های تحریک کننده اینترفرون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
2â²-5â²-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein that exerts antiviral effects through the RNase L- or retinoic acid-inducible gene I (RIG-I)-dependent signalling pathway. In this study, we identified and cloned the OASL gene (named duOASL) from healthy adult Cherry Valley ducks. Full-length duOASL cDNA (1630Â bp) encoded a 504-amino acid polypeptide containing three conserved domains, namely, nucleotidyltransferase domain, 2â²-5â²-oligoadenylate synthetase domain, and two ubiquitin-like repeats. DuOASL mRNA expression was quantified by performing quantitative reverse transcription-PCR (qRT-PCR). Results of qRT-PCR showed that duOASL was broadly expressed in all examined tissues, with the highest mRNA expression in the large intestine. Antiviral activity of duOASL was measured by determining its effect on Duck Tembusu virus (DTMUV) replication in vitro. We found that duOASL overexpression slightly inhibited DTMUV replication, whereas duOASL knockdown by using a specific small interfering RNA increased DTMUV replication in DF-1 cells. Thus, we successfully cloned and characterized the antiviral protein duOASL from Cherry Valley ducks and found that it exerted antiviral effects against DTMUV. These results provide a solid foundation for performing further studies to determine the mechanism underlying the antiviral effect of duOASL at the cellular level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 629, 20 September 2017, Pages 43-51
Journal: Gene - Volume 629, 20 September 2017, Pages 43-51
نویسندگان
Ke-Ran Bi, Kai-Kai Han, Qing-Tao Liu, Dong-Min Zhao, Xin-Mei Huang, Yu-Zhuo Liu, Jing Yang, Yin Li,