کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5591421 | 1570396 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Creatine kinase in human erythrocytes: A genetic anomaly reveals presence of soluble brain-type isoform
ترجمه فارسی عنوان
کراتین کیناز در اریتروسیت های انسانی: یک آنومالی ژنتیکی نشان می دهد وجود ایزوفرم نوعی محلول در مغز
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
چکیده انگلیسی
For maintaining energy homeostasis, creatine kinase (CK) is present at elevated levels in tissues with high and/or fluctuating energy requirements such as muscle, brain, and epithelia, while there is very few CK, if any, in peripheral blood cells. However, an ectopic expression of brain-type creatine kinase (BCK) has been reported for platelets and leukocytes in an autosomal dominant inherited anomaly named CKBE. Here we investigated CK in erythrocytes of CKBE individuals from eight unrelated families. The data revealed a varying but significant increase of CK activity in CKBE individuals as compared to controls, reaching an almost 800-fold increase in two CKBE individuals which also had increased erythrocyte creatine. Immunoblotting with highly specific antibodies confirmed that the expressed CK isoform is BCK. Cell fractionation evidenced soluble BCK, suggesting cytosolic and not membrane localization of erythrocyte CK as reported earlier. These results are discussed in the context of putative CK energy buffering and transfer functions in red blood cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Blood Cells, Molecules, and Diseases - Volume 64, May 2017, Pages 33-37
Journal: Blood Cells, Molecules, and Diseases - Volume 64, May 2017, Pages 33-37
نویسندگان
Laurence Kay, Malgorzata Tokarska-Schlattner, Bénédicte Quenot-Carrias, Betty Goudet, Peter Bugert, Heidwolf Arnold, Günter Scheuerbrandt, Uwe Schlattner,