|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5593421||1571080||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
- An icing protocol of the vastus lateralis after cycling was performed.
- PGC-1Î±, Tfam, NRF1, NRF2, and ERRÎ± mRNA were analyzed.
- No differences occurred in gene expression between the iced limb and control.
- Icing does not alter gene expression related to mitochondrial development.
Recovery that takes place in a cold environment after endurance exercise elevates PGC-1Î± mRNA whereas ERRÎ± and NRF2 mRNA expression are inhibited. However, the effect of local skeletal muscle cooling on mitochondrial-related gene expression is unknown.PurposeTo determine the impact of local skeletal muscle cooling during recovery from an acute bout of exercise on mitochondrial-related gene expression.MethodsRecreationally-trained male cyclists (n=8, age 25Â±3 y, height 181Â±6Â cm, weight 79Â±8Â kg, 12.8Â±3.6% body fat, VO2peak 4.52Â±0.88Â LÂ·minâ1 protocol) completed a 90-min variable intensity cycling protocol followed by 4Â h of recovery. During recovery, ice was applied intermittently to one leg (ICE) while the other leg served as a control (CON). Intramuscular temperature was recorded continuously. Muscle biopsies were taken from each vastus lateralis at 4Â h post-exercise for the analysis of mitochondrial-related gene expression.ResultsIntramuscular temperature was colder in ICE (26.7Â±1.1Â Â°C) than CON (35.5Â±0.1Â Â°C) throughout the 4Â h recovery period (p<0.001). There were no differences in expression of PGC-1Î±, TFAM, NRF1, NRF2, or ERRÎ± mRNA between ICE and CON after the 4Â h recovery period.ConclusionLocal muscle cooling after exercise does not impact the expression of mitochondrial biogenesis-related genes compared to recovery from exercise in control conditions. When these data are considered with previous research, the stimuli for cold-induced gene expression alterations may be related to factors other than local muscle temperature. Additionally, different intramuscular temperatures should be examined to determine dose-response of mitochondrial-related gene expression.
Journal: Journal of Thermal Biology - Volume 67, July 2017, Pages 35-39