کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5638060 | 1583274 | 2017 | 5 صفحه PDF | دانلود رایگان |
- A total of 36 eight-week-old male C57BL6/J mice were used in this experiment.
- There was an increase in the number of osteoblasts (p Ë 0.05) in both the Vehicle and Probiotic groups on the right side (under OTM).
- Probiotic therapy for 12Â days decreased the number of osteoclasts.
AimsThe aim of the present study was to investigate the effect of probiotic (Bacillus Subtilis) supplementation on bone remodelling induced by mechanical loading.MethodsC57BL/6 mice were divided in two groups: (1) Probiotic and (2) Vehicle (water). The probiotic (1.5 Ã 108 CFU/mL) was administered orally for 14 days, starting two days before the induction of orthodontic tooth movement (OTM). OTM was determined by histomorphometric analysis by comparing the right to the left side of the maxilla. The number of osteoclasts was determined by counting TRAP-positive cells. Osteoblasts were counted on Masson's trichrome-stained slides.ResultsOTM was similar between groups (with and without probiotic supplementation) (p = 0.46). The number of TRAP-positive cells increased (p < 0.01) on the experimental side (where the spring coil was installed) in comparison to the control side in both groups. However, the number of osteoclasts decreased (p Ë 0.01) in the probiotic group, in comparison to the vehicle group. There was an increase in the number of osteoblasts (p Ë 0.05) in both the Vehicle and Probiotic groups on the side under OTM, independent of probiotic supplementation.ConclusionOral Supplementation with a probiotic influenced the number of osteoclasts adjacent to the tooth root during orthodontic movement in mice.
Journal: Archives of Oral Biology - Volume 79, July 2017, Pages 30-34