کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5657846 1407412 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Growth differentiation factor 15 promotes cell viability, invasion, migration, and angiogenesis in human liver carcinoma cell line HepG2
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های گوارشی
پیش نمایش صفحه اول مقاله
Growth differentiation factor 15 promotes cell viability, invasion, migration, and angiogenesis in human liver carcinoma cell line HepG2
چکیده انگلیسی

SummaryAimThis study was aimed to explore the role of growth differentiation factor 15 (GDF15) in hepatocellular carcinoma (HCC).MethodsHuman liver carcinoma cell line HepG2 was used and transfected with vector and/or short hairpin RNA (shRNA) against GDF15. Then, the transfection efficiency was ascertained by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Cell viability was measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide (MTT). Cell invasion and migration were measured by Transwell assay and Scratch assay. In addition, human umbilical vein endothelial cell (HUVEC) tube formation assay was performed to analysis the angiogenesis. Further, the protein expressions of epithelial-mesenchymal transition (EMT)-related factors were measured by Western blot.ResultWe found that GDF15 overexpression significantly facilitated cell viability, cell invasion, migration, and angiogenesis (P < 0.05 or P < 0.01). The protein expressions of N-Cadherin, Vimentin and Twist1 were up-regulated by GDF15 overexpression, while E-Cadherin was down-regulated. Reciprocally, using a GDF15-shRNA strategy, we observed that GDF15 downregulation inhibited both basal and GDF15-induced cell viability, migration, invasion and angiogenesis in HepG2 cells.ConclusionGDF15 could promote cell viability, invasion, migration, and angiogenesis of HepG2 cells. GDF15 overexpression might be a potential risk factor of HCC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinics and Research in Hepatology and Gastroenterology - Volume 41, Issue 4, September 2017, Pages 408-414
نویسندگان
, , , ,