کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5665779 | 1407771 | 2017 | 4 صفحه PDF | دانلود رایگان |
- Agreement with the manufacturer's methodology was >90% for TB first line drugs.
- Subculturing of M. tuberculosis isolates in MGIT as recommended can be excluded.
- Modified protocol is more rapid and has lower cost than manufacturer's methodology.
A rapid detection of resistance in Mycobacterium tuberculosis is crucial for management and control of tuberculosis. This study evaluated a more rapid and cost-effective drug susceptibility testing (DST) protocol using primary isolates of M. tuberculosis in mycobacteria growth indicator tube (MGIT). Ninety-four M. tuberculosis isolates in MGIT were subjected to DST by the manufacturer's method, i.e., primary isolates were subcultured and DST was performed from positive cultures for a maximum of 5Â days; and by our modified method, i.e., DST was performed directly from primary MGIT cultures positive for more than 5Â days. Results were concordant for 76 (81%) isolates. Agreement between both methods was 92.0%, 98.9%, 97.7%, and 95.5% for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. Six isolates failed to grow on the recommended method, including 3 resistant isolates. Not performing subculture of primary M. tuberculosis isolates yields reliable results, decreasing the turnaround time and the cost of the test.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 2, February 2017, Pages 108-111