کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665867 1407775 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection
ترجمه فارسی عنوان
ارزیابی روش تشخیصی سریع و معقول برای تشخیص دقیق عفونت حاد دنگی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- Dengue Day 1 rapid diagnostic test is a simple, point-of-care test for dengue.
- Exhibits excellent performance for diagnosis of acute dengue infection in children.
- Shows higher sensitivity compared to NS1 ELISA.
- Detects all four dengue serotypes.

The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 3, March 2017, Pages 229-234
نویسندگان
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