Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system

- Development of multiplex real-time PCR assay on commercial BD MAX open system platform.
- BD MAX is a fully automated system for all PCR processes including sample extraction to PCR.
- Assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 carbapenemase gene families.
- A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates.
- This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.

A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.