In vitro antiprotozoal activity of (S)-cis-Verbenol against Leishmania spp. and Trypanosoma cruzi

- (S)-cis-Verbenol showed good activity against Leishmania spp. and strains of Trypanosoma cruzi.
- The compound showed better selectivity than the reference drugs, amphotericin B and Benznidazole.
- (S)-cis-Verbenol activity was not significantly different for the Leishmania spp assayed.
- The compound activity showed significant differences against CL Brener and Ypsilon (Y) strains of T. cruzi.

(S)-cis-Verbenol, a monoterpene frequently found as a component of essential oils, was assayed against Leishmania amazonensis, Leishmania infantum, Leishmania brasiliensis and against two strains of Trypanosoma cruzi. The cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. Benznidazole was used as reference drug against T. cruzi and amphotericin B was used against Leishmania spp. The compound showed good activity against the trypanosomes, being more active against the CL Brenner strain, with an IC50 value of 8.3 μg/mL. Against Leishmania, the IC50 values were between 2.1 and 3.8 μg/mL. The compound showed no cytotoxicity against human fibroblasts at the concentrations assayed and was 100-500 times more toxic for the parasites than for the human cells, as indicated by the selectivity indexes. The results open interesting perspectives about the potential of (S)-cis-Verbenol and other individual components of essential oils for the treatment of these diseases.

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