کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5672798 | 1408124 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison among four proposed direct blood culture microbial identification methods using MALDI-TOF MS
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماری های عفونی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, we assessed the accuracy in identification of both Gram-negative and Gram-positive bacteria, except for Streptococcus viridans, using four rapid blood culture methods with Vitek MALDI-TOF-MS. We compared our proposed lysis centrifugation followed by washing and 30% acetic acid treatment method (method 2) with two other lysis centrifugation methods (washing and 30% formic acid treatment (method 1); 100% ethanol treatment (method 3)), and picking colonies from 90 to 180Â min subculture plates (method 4). Methods 1 and 2 identified all organisms down to species level with 100% accuracy, except for Streptococcus viridans, Streptococcus pyogenes, Enterobacter cloacae and Proteus vulgaris. The latter two were identified to genus level with 100% accuracy. Each method exhibited excellent accuracy and precision in terms of identification to genus level with certain limitations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Infection and Public Health - Volume 10, Issue 3, MayâJune 2017, Pages 308-315
Journal: Journal of Infection and Public Health - Volume 10, Issue 3, MayâJune 2017, Pages 308-315
نویسندگان
Ali M. Bazzi, Ali A. Rabaan, Zeyad El Edaily, Susan John, Mahmoud M. Fawarah, Jaffar A. Al-Tawfiq,