کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5673625 1593678 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical composition and modulation of bacterial drug resistance of the essential oil from leaves of Croton grewioides
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Chemical composition and modulation of bacterial drug resistance of the essential oil from leaves of Croton grewioides
چکیده انگلیسی


- The essential oil and α-pinene had a synergistic effect when associated with the antibiotics.
- Essential oil exhibited the best result.
- Reducing the MIC of norfloxacine and tetracicline, essential oil, α-pinene are consider as a putative efflux pump inhibitor.

The essential oil from leaves of Croton grewioides Baill was obtained by hydrodistillation using Clevenger apparatus, and its chemical composition was analyzed by GC-MS, where 18 compounds were identified, mostly as monoterpenes (55.56%) and sesquiterpenes (44.44%), in which the major constituent was the α-pinene (47.43%). The essential oil of Croton grewioides (EOCg) and its major compound (α-pinene) were evaluated as modulators of antibiotic resistance in strain SA-1199B and IS-58 of Staphylococcus aureus that overexpresses efflux protein. The minimum inhibitory concentrations (MICs) of the antibiotics were determined by the microdilution assay in the absence and in the presence of sub-inhibitory concentration of EOCg and α-pinene. Although the EOCg and α-pinene did not indicate relevant antibacterial activity in vitro, they acted as antibiotic resistance modulators, i.e., EOCg in combination with norfloxacin, reducted its MIC, by 64× whereas in combination with tetracycline it was observed a reduction of 4×. Additionally, it was observed a MIC reduction of tetracycline by 32×, when combined with α-pinene. The results suggest that EOCg and α-pinene modulate or even reverse bacterial resistance as a putative efflux pump inhibitor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 111, October 2017, Pages 468-471
نویسندگان
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