کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5673995 1593685 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cholesterol and ergosterol affect the activity of Staphylococcus aureus antibiotic efflux pumps
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Cholesterol and ergosterol affect the activity of Staphylococcus aureus antibiotic efflux pumps
چکیده انگلیسی
The aim of this study is to evaluate the effect of ergosterol on steroids and cholesterol efflux pumps in multidrug resistant strains of S. aureus. Were used RN4220 harboring plasmid pUL5054, which carries the gene encoding the MsrA macrolide efflux protein; and IS-58, which possesses the TetK tetracycline efflux protein; 1199B resists hydrophilic fluoroquinolones via a NorA-mediated mechanism and wild strain 1199B. The Minimal Inhibitory Concentration (MIC) was determined and the evaluation of possible inhibition of efflux pumps by reduction of MIC. Some of the detrimental effects on bacterial cells can be attributed to the detergent properties of cholesterol and ergosterol on account of their amphipathic structure. Besides the cholesterol did not affect directly the pump structure, a synergism was observed, maybe due the interaction with the cell membrane and interference in the lipid bilayer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 104, March 2017, Pages 133-136
نویسندگان
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