کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5675231 1594207 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cholesterol is required for stability and infectivity of influenza A and respiratory syncytial viruses
ترجمه فارسی عنوان
کلسترول برای ثبات و ابتلا به آنفلوآنزا و ویروس های سونسیتیال تنفسی مورد نیاز است
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- Cholesterol-reducing agents do not inhibit production of IAV or RSV.
- Progeny IAV and RSV released from the treated cells are unstable.
- Cholesterol is essential for sustaining viral stability and infectivity.

Cholesterol-rich lipid raft microdomains in the plasma membrane are considered to play a major role in the enveloped virus lifecycle. However, the functional role of cholesterol in assembly, infectivity and stability of respiratory RNA viruses is not fully understood. We previously reported that depletion of cellular cholesterol by cholesterol-reducing agents decreased production of human parainfluenza virus type 1 (hPIV1) particles by inhibiting virus assembly. In this study, we analyzed the role of cholesterol on influenza A virus (IAV) and respiratory syncytial virus (RSV) production. Unlike hPIV1, treatment of human airway cells with the agents did not decrease virus particle production. However, the released virions were less homogeneous in density and unstable. Addition of exogenous cholesterol to the released virions restored virus stability and infectivity. Collectively, these data indicate a critical role of cholesterol in maintaining IAV and RSV membrane structure that is essential for sustaining viral stability and infectivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 510, October 2017, Pages 234-241
نویسندگان
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