کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5675363 1594319 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Baculovirus LEF-11 nuclear localization signal is important for viral DNA replication
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Baculovirus LEF-11 nuclear localization signal is important for viral DNA replication
چکیده انگلیسی


- Baculovirus LEF-11 contains monopartite and bipartite classical nuclear localization signal sequence (cNLSs).
- BmNPV LEF-11 enters the nucleus during virus infection in the absence of an NLS.
- The NLS of LEF-11 is involved in virus DNA replication.

Baculovirus LEF-11 is a small nuclear protein that is involved in viral late gene transcription and DNA replication. However, the characteristics of its nuclear localization signal and its impact on viral DNA replication are unknown. In the present study, systemic bioinformatics analysis showed that the baculovirus LEF-11 contains monopartite and bipartite classical nuclear localization signal sequences (cNLSs), which were also detected in a few alphabaculovirus species. Localization of representative LEF-11 proteins of four baculovirus genera indicated that the nuclear localization characteristics of baculovirus LEF-11 coincided with the predicted results. Moreover, Bombyx mori nucleopolyhedrovirus (BmNPV) LEF-11 could be transported into the nucleus during viral infection in the absence of a cNLSs. Further investigations demonstrated that the NLS of BmNPV LEF-11 is important for viral DNA replication. The findings of the present study indicate that the characteristics of the baculovirus LEF-11 protein and the NLS is essential to virus DNA replication and nuclear transport mechanisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 238, 15 June 2017, Pages 133-140
نویسندگان
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