کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5735409 1612900 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportDevelopment of an opioid self-administration assay to study drug seeking in zebrafish
ترجمه فارسی عنوان
گزارش تحقیق توسعه یک روش خودکارآمدی از مواد مخدر برای مطالعه مواد مخدر در کوره های سفید
کلمات کلیدی
اعتیاد، اپوئید، ماهی قزل آلا، خودمختاری، مدل حیوانی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
چکیده انگلیسی


- Zebrafish can be conditioned to self-administer opioids.
- Conditioned fish developed several hallmarks of addiction.
- Self-administration in zebrafish relies on the same molecular pathways utilized in other animal models.

The zebrafish (Danio rerio) has become an excellent tool to study mental health disorders, due to its physiological and genetic similarity to humans, ease of genetic manipulation, and feasibility of small molecule screening. Zebrafish have been shown to exhibit characteristics of addiction to drugs of abuse in non-contingent assays, including conditioned place preference, but contingent assays have been limited to a single assay for alcohol consumption. Using inexpensive electronic, mechanical, and optical components, we developed an automated opioid self-administration assay for zebrafish, enabling us to measure drug seeking and gain insight into the underlying biological pathways. Zebrafish trained in the assay for five days exhibited robust self-administration, which was dependent on the function of the μ-opioid receptor. In addition, a progressive ratio protocol was used to test conditioned animals for motivation. Furthermore, conditioned fish continued to seek the drug despite an adverse consequence and showed signs of stress and anxiety upon withdrawal of the drug. Finally, we validated our assay by confirming that self-administration in zebrafish is dependent on several of the same molecular pathways as in other animal models. Given the ease and throughput of this assay, it will enable identification of important biological pathways regulating drug seeking and could lead to the development of new therapeutic molecules to treat addiction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 335, 29 September 2017, Pages 158-166
نویسندگان
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