کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5738475 1615056 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research articleEndocytosis contributes to BMP2-induced Smad signalling and neuronal growth
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research articleEndocytosis contributes to BMP2-induced Smad signalling and neuronal growth
چکیده انگلیسی


- Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth.
- Dynasore attenuates canonical BMPR-Smad 1/5/8 signalling following BMP2 treatment.
- Dynamin-dependent endocytosis inhibition delays BMP2-activation of Smad signalling.
- Dynasore inhibits BMP2-induced increases of SH-SY5Y neurite length and branching.

Bone morphogenetic protein 2 (BMP2) is a neurotrophic factor which induces the growth of midbrain dopaminergic (DA) neurons in vitro and in vivo, and its neurotrophic effects have been shown to be dependent on activation of BMP receptors (BMPRs) and Smad 1/5/8 signalling. However, the precise intracellular cascades that regulate BMP2-BMPR-Smad-signalling-induced neurite growth remain unknown. Endocytosis has been shown to regulate Smad 1/5/8 signalling and differentiation induced by BMPs. However, these studies were carried out in non-neural cells. Indeed, there are scant reports regarding the role of endocytosis in BMP-Smad signalling in neurons. To address this, and to further characterise the mechanisms regulating the neurotrophic effects of BMP2, the present study examined the role of dynamin-dependent endocytosis in BMP2-induced Smad signalling and neurite growth in the SH-SY5Y neuronal cell line. The activation, temporal kinetics and magnitude of Smad 1/5/8 signalling induced by BMP2 were significantly attenuated by dynasore-mediated inhibition of endocytosis in SH-SY5Y cells. Furthermore, BMP2-induced increases in neurite length and neurite branching in SH-SY5Y cells were significantly reduced following inhibition of dynamin-dependent endocytosis using dynasore. This study demonstrates that BMP2-induced Smad signalling and neurite growth is regulated by dynamin-dependent endocytosis in a model of human midbrain dopaminergic neurons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 643, 16 March 2017, Pages 32-37
نویسندگان
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