|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5740083||1412156||2018||13 صفحه PDF||ندارد||دانلود کنید|
â¢Identification of a P.Â expansum transcription factor Stel2.â¢PeSte12 is relevant for virulence and asexual reproduction.â¢Reduction in virulence by silencing Ste12 gene affect P.Â expansum H2O2 suppression.â¢PeSte12 is induced at medium stages of apple infection.â¢PeSte12 acts mostly as negative regulator during inÂ vitro growth.
Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0Â Â°C. The ÎPeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the ÎPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain.Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in ÎPeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P.Â expansum's virulence and asexual reproduction.
Journal: Food Microbiology - Volume 69, February 2018, Pages 123-135