کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5740369 | 1616295 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biofilm communities survey at the areas of salt crystallization on the walls of a decorated shelter listed at UNESCO World cultural Heritage
ترجمه فارسی عنوان
بررسی جوامع بیوفیلم در مناطق کریستالیزاسیون نمک بر روی دیوارهای یک پناهگاه تزئین شده در فهرست میراث جهانی یونسکو
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کلمات کلیدی
کاهش وزن بیوفیلم، تنوع میکروبی، پناه، گچ،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
علوم زیست محیطی (عمومی)
چکیده انگلیسی
The microbial diversity on the walls of the UNESCO World cultural Heritage site Laugerie-Haute east shelter, France, was surveyed in a context of degradation by formation of salt efflorescences. Samples of salt efflorescence and underlying rock were collected in autumn and spring. Sessile microorganisms were studied by cultivation, microscopy, and molecular methods. Concentrations of the order of 103 fungal and 107 bacterial cells per gram of sample were quantified by culturing at both seasons. Higher concentrations (107 to 109Â cells per gram) of sessile microorganisms were determined by microscopic counting after DAPI staining with a significant increase in sessile cells in spring. The presence of biofilms was confirmed by electron microscopy with the observation of microorganisms embedded in an abundant matrix in association with rock. Members of Firmicutes, Actinobacteria, and Ascomycota were identified among the cultivable sessile microflora. Bacillus herbersteinensis and Engyodontium album were the only microorganisms to be isolated in both seasons. Sequence analysis of 16S rRNA genes and internal transcribed spacer clone libraries showed dominance of Bacteroidetes, Proteobacteria, and Ascomycota. Bacteroidetes and Actinobacteria were more abundant in autumn, and in spring, respectively. Cyanobacteria were present at both seasons. Bacterial richness increased during spring. Whatever the season, clones affiliated to the 4 bacterial genera Aureimonas, Sphingopyxis, Halomonas, and Crossiella, and to the two fungal genera Engyodontium and Teratosphaeria were present. In conclusion, we demonstrated the presence of abundant biofilm at efflorescence salt on the walls of the shelter; that was increased in quantity and diversity in spring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 122, August 2017, Pages 116-127
Journal: International Biodeterioration & Biodegradation - Volume 122, August 2017, Pages 116-127
نویسندگان
Clémentine Lepinay, Agnès Mihajlovski, Damien Seyer, Stéphanie Touron, Faisl Bousta, Patrick Di Martino,