کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5766966 1628208 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A characterization of structural proteins expressed by Bombyx mori bidensovirus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
A characterization of structural proteins expressed by Bombyx mori bidensovirus
چکیده انگلیسی


- BmBDV VP uses a leaking scanning translation mechanism to express products of 3 different molecular weights.
- BmBDV SP is confirmed to be a structural protein and a part of virus particles.
- Different sites and degrees of VP oxidation modification are identified.

Bombyx mori bidensiovirus (BmBDV) is a species of Bidensovirus that has been was placed into a new genus within the new family Bidnaviridae by the International Committee on Taxonomy of Viruses. BmBDV causes fatal flacherie disease in silkworms, which causes large losses to the sericulture industry. BmBDV contains two sets of complementary linear single-stranded DNAs of approximately 6.5 kb (viral DNA 1, VD1) and 6.0 kb (viral DNA 2, VD2). VD1 and VD2 are encapsidated in separate icosahedral non-enveloped capsids, which are similar in size and shape. However, the strategies used to express BmBDV structural proteins remains unclear. In this work, a total of six structural proteins were separated by two-dimensional electrophoresis and shown to be encoded by the BmBDV VP gene via mass spectrometry. The transmission electron microscopy results showed that co-expression of the BmBDV VP and SP structural proteins in Spodoptera frugiperda sf9 cells resulted in the formation of 22-24 nm virus-like particles. Furthermore, a mutation of the major structural protein-encoding VP gene, in which the second in-frame ATG codon was mutated to GCG, abrogated the production of several structural proteins, indicating that this strategy of expressing BmBDV VP is dependent on a leaky scanning translation mechanism.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Invertebrate Pathology - Volume 144, March 2017, Pages 18-23
نویسندگان
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