کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5767524 1628386 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid quantification of cholesterol in dairy powders using Fourier transform near infrared spectroscopy and chemometrics
ترجمه فارسی عنوان
اندازه گیری سریع کلسترول در پودرهای لبنی با استفاده از تبدیل فوریه در نزدیکی طیف سنجی مادون قرمز و شیمی درمانی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- FT-NIR spectroscopy used for cholesterol quantification from dairy powders using 80 samples.
- Major peaks of cholesterol successfully elucidated.
- Second derivative preprocessing gave the best predictive ability with highest r2 of 0.9998 and lowest RMSECV of 1.05.
- Comparison with HPLC method proved its accuracy and reproducibility.

A rapid method for the quantification of cholesterol in the standardized dairy powders was developed using Fourier transform near-infrared (FT-NIR) spectroscopy coupled with appropriate chemometric techniques. Different spectral preprocessing methods were investigated for the partial least-squares (PLS) regression model development. The results showed that the second derivative PLS model in the spectral region of 6101.9-5446.2 cm−1 was the most robust with the best performance indicators (r2 validation = 0.9998, RMSECV = 1.05 mg cholesterol/100 g, rank = 6 and RPD > 8). Functional band assignment of the major spectral peaks in the cholesterol spectrum was also possible. Statistical evaluation with the HPLC method proved that the developed NIR-chemometric method has good reproducibility and satisfactory accuracy profile. The comparable relative standard deviation (RSD) along with good precision accuracy (95.9-101%) of the proposed FT-NIR method, demonstrate its suitability for the rapid and routine analysis of cholesterol content in the dairy powders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 78, August 2017, Pages 342-349
نویسندگان
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