کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5767764 | 1628449 | 2017 | 7 صفحه PDF | دانلود رایگان |
- We examined the presence of the (â)-epigallocatechin moiety in fresh tea leaves.
- LC-MS confirmed the presence of EGCpCA and EGCCA.
- EGCCA exhibited a stronger antioxidant activity than EGCG in the ORAC assay.
- Analysis and quantification is possible using MRM and natural compound standards.
(â)-Epigallocatechin-3-O-gallate (EGCG), the major catechin present in green tea, exhibits potent antioxidant activity. We thereby investigated the presence of unknown components bearing the (â)-epigallocatechin (EGC) moiety in fresh tea leaf samples. Initially, liquid chromatography tandem mass spectrometry (LC-MS/MS) was employed to examine fresh tea leaves of the Yabukita, the most popular tea cultivar in Japan, which suggested the presence of the EGC phenylpropanoid derivatives, (â)-epigallocatechin-3-O-p-coumaroate (EGCpCA) and (â)-epigallocatechin-3-O-caffeoate (EGCCA). The structures of the detected EGCpCA and EGCCA were then confirmed by LC-MS/MS using synthesized EGCpCA and EGCCA as standards. In addition, EGCpCA and EGCCA were evaluated for their antioxidant activity in the ORAC (oxygen radical antioxidant capacity) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assays, where EGCCA (8.60 μmol TE/μmol, TE = Trolox equivalents) exhibited a stronger antioxidant activity than EGCG (5.52 μmol TE/μmol) in the ORAC assay. Finally, EGCpCA and EGCCA were quantitated in several tea leaf samples using LC-MS/MS, and it was found that these compounds were present in lower quantities (EGCpCA, 16.8-345.8 μg/g, EGCCA, 4.3-75.1 μg/g in the dry tea leaves) than the major catechins. In this study, we found the potent antioxidant EGCCA using LC-MS/MS and revealed its wide existence in various tea leaves.
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Journal: Food Research International - Volume 102, December 2017, Pages 77-83