Research PaperSoil-applied phosphorous is an effective tool to mitigate the toxicity of copper excess on grapevine grown in rhizobox

- Grapevines were grown at increasing rate of Cu (0-300 ppm) and P (0-100 ppm).
- Shoot growth and plant biomass declined with the increase of soil Cu concentration.
- The application of P enhanced plant growth and nutritional status.
- Phosphorous stimulated root length, diameter and the number of apex.
- Root citric acid increased in Cu300, while ascorbic acid decreased in Cu 200 and 300.

The aim of the present study was to evaluate the effectiveness of P soil addition to mitigate the toxic effect of excess of Cu on grapevine on sandy non-calcareous soil.Micro-propagated plants of 1103 Paulsen rootstock were grown according to a factorial experimental design with two factors: Cu addition (0, 100, 200 and 300 mg kg−1 dw) and P addition (0, 50 and 100 mg kg−1 soil dw). The experiment was conducted in the greenhouse and plants were grown on a sandy soil in rhizoboxes. At the end of the trial, plants were destructively harvested, the biomass and the nutrient concentration of organs were determined. Root growth was evaluated during the trial and the concentration of organic acid in root tissue and exudates was determined.Shoot length and plant biomass declined as Cu concentration in soil increased; the application of P enhanced plant growth and nutritional status. Root biomass decreased at Cu > 100 mg kg−1 and increased in P at 100 mg kg−1. Phosphorous stimulated root length, diameter and the number of apexes. Root citric acid increased as a response of Cu at 300 mg kg−1, root ascorbic acid decreased with the increase of Cu (200 and 300 mg kg−1) and P at 100 mg kg−1. The concentration of citric acid from root exudations was higher in Cu at 300 mg kg−1 and Cu at 200 mg kg−1 (compared to control) and P at 100 mg kg−1 soils.The application of P fertilization seems to be a valuable strategy to overcome the toxicity of high concentration of Cu in soil.