کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5791243 | 1554088 | 2015 | 6 صفحه PDF | دانلود رایگان |
- A sensitive HPLC method for the determination of RAC in pork samples with low CCβ
- Extraction of ractopamine residue by liquid/liquid extraction, SPE and derivatization
- Separation of ractopamine derivative with TFA as an ion-pairing reagent
Ractopamine (RAC) has been approved as a feed additive for swine, cattle or turkey, and is likely to have residue in edible animal products and may pose a potential risk for consumer health. Therefore, it is essential to establish a method to detect the residue of RAC in animal products. This work presents a rapid and sensitive HPLC method for the determination of RAC in pork samples with pre-column derivatization. The RAC derivative was separated on a kromasil C18 column and detected at 284 nm with a UV detector. The detection capability (CCβ) was 0.078 μg gâ 1 and the linearity was established over the concentration range of 0.15-100.0 μg gâ 1. The overall mean recovery in spike range of 0.2 μg gâ 1 to 100 μg gâ 1 was 89.9% with the overall mean relative standard deviation of 4.1%. This method can be used for the quantification of RAC in pork samples and help to establish adequate monitoring of the residue of RAC.
Journal: Meat Science - Volume 106, August 2015, Pages 55-60