کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5795401 1554351 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytokines expression in alpacas and llamas exposed to cold stress
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Cytokines expression in alpacas and llamas exposed to cold stress
چکیده انگلیسی


- Cytokine expression was measured from alpacas and llamas with pneumonia.
- IL-1β expression is greater in camelids exposed to low temperatures.
- Pasteurella multocida induce cytokine expression in healthy alpaca monocytes.

Cytokine expression could play an important role in camelids exposed to cold stress and subsequent pneumonia associated infections, therefore inducing a broad array of inflammatory mediators. To understand the host innate immune response in camelids exposed to cold weather, IL-1α, IL-1β, IL-6 and TNFα mRNA expression were examined using quantitative real-time polymerase chain reaction method using Syber Green PCR master mix in peripheral blood from lung of alpacas and llamas during the cold season in Perú. Results demonstrated that all studied cytokines but IL-1α had greater level of expression, and that IL-1β expression was significant greater in camelids exposed to cold weather and living at high altitude compared to alpacas living in mild temperatures and at sea level. Cytokines expression was minimal in the group of alpacas living at sea level. All lung tissue samples from the camelids exposed to low temperatures and living at high altitude were associated with a degree of pneumonia by histopathological examination. Exposure of Pasteurella multocida to healthy alpaca monocytes showed greater IL-1β cytokine expression among the cytokines studied here. These cytokines might participate in the immune and inflammatory responses against adverse weather conditions and during the pulmonary defense mechanisms against pathogens infection in alpacas and llamas. These findings provide important information for the development of a preventable treatment programs and appropriate conditions for welfare in camelids.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 141, August 2016, Pages 135-140
نویسندگان
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