Development and characterization of monoclonal antibodies against goose CD3ɛ extracellular domain and their application in detection of CD3+ T lymphocytes

- MAbs against goose CD3ɛ extracellular domain was produced and characterized for the first time.
- MAb 3C11 and 5A3 had excellent immunoreactivity with rGoCD3ɛex, peripheral blood lymphocytes and the polypeptide of goose CD3ɛ expressed in the eukaryotic cells.
- MAb 3C11 and 5A3 could be used to detect the percentage and shape of CD3+ T lymphocytes.

CD3 is one of the most important cell surface markers of T lymphocytes, and has an important function in signal transmission during antigen recognition. In this study, monoclonal antibodies against goose CD3ɛ extracellular domain were developed and characterized for the first time. The purified recombinant goose CD3ɛ extracellular domain protein and pcDNA3.1-GoCD3ɛex were used as immunogens to develop mAbs. Furthermore, recombinant protein was used to screen for mAbs. The characteristics of mAbs were identified through IFA, I-ELISA, Western blot, FCM, and LSCM analyzes. Results showed that mAbs 3C11 and 5A3 may be good candidates for use in detecting goose T lymphocytes and in immunoassaying and exploring the function of the CD3ɛ molecule.