کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5796711 1555017 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histamine levels in embryonic chicken livers infected with very virulent infectious bursal disease virus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Histamine levels in embryonic chicken livers infected with very virulent infectious bursal disease virus
چکیده انگلیسی

Histamine is an endogenous nitrogenous compound with extensive effects on immunologic cells and involved in many physiological functions. The current aim was to determine histamine levels in embryonic liver and its association with the pathogenicity of a very virulent infectious bursal disease virus (vvIBDV) isolate serially passaged in chicken embryos. A vvIBDV isolate and the passaged viruses were inoculated into SPF embryonated chicken eggs (0.2 ml per egg) via the chorioallantoic membrane. Embryonic livers were collected at 24, 48, 72, 96, and 120 h post-inoculation and histamine contents were quantified by fluorescence spectrophotometry analyses. Results showed that the histamine content in embryonic livers infected with the original vvIBDV isolate and the early passaged viruses significantly increased 48 h post-inoculation, as compared with the adapted IBDV isolate (p < 0.01) and controls (p < 0.01), with the concentration peaking from 72 h to 96 h. Most of the infected chicken embryos died from 48 h to 96 h post-inoculation. Moreover, the histamine content in dead embryos was markedly increased compared with live embryos (p < 0.05), peaking at 72 h post-inoculation (p < 0.01). There was an association between histamine content in embryonic livers and an elevation in histidine decarboxylase activity. Taken together, our results suggest that an excess of histamine correlates with inflammatory responses during vvIBDV infection. This study provides an incremental step in the understanding of the pathogenesis of vvIBDV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 168, Issues 1–2, 15 November 2015, Pages 91-96
نویسندگان
, , , , , ,