کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5800003 | 1555352 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene](/preview/png/5800003.png)
- Two newly HP-PRRSV) strains with GP2 deletion were isolated.
- Significant variation occurs in their genome.
- The deletion did not affect viral growth in MARC-145 cells, GP2 may be variable.
Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable.
Journal: Veterinary Microbiology - Volume 176, Issues 3â4, 17 April 2015, Pages 344-351