کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800003 1555352 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene
چکیده انگلیسی


- Two newly HP-PRRSV) strains with GP2 deletion were isolated.
- Significant variation occurs in their genome.
- The deletion did not affect viral growth in MARC-145 cells, GP2 may be variable.

Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 176, Issues 3–4, 17 April 2015, Pages 344-351
نویسندگان
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