کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5827343 | 1558923 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Melatonin affects voltage-dependent calcium and potassium currents in MCF-7 cell line cultured either in growth or differentiation medium
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کلمات کلیدی
α-DTXNifedipine (PubChem CID: 4485)Melatonin (PubChem CID: 896)MT1MLTT-type Ca2+ currentChrIBKiberiotoxinIbTXRMPMTSIKSICa,L - ICA، LIKCa - IKC بهL-type Ca2+ current - L-type Ca2 + currentα-dendrotoxin - α-دندروتوکسینElectrophysiology - الکتروفیزیولوژیholding potential - داشتن پتانسیلMelatonin - ملاتونینresting membrane potential - پتانسیل غشای استراحتChromanol - کرومونول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
Big efforts have been dedicated up to now to identify novel targets for cancer treatment. The peculiar biophysical profile and the atypical ionic channels activity shown by diverse types of human cancers suggest that ion channels may be possible targets in cancer therapy. Earlier studies have shown that melatonin exerts an oncostatic action on different tumors. In particular, it was shown that melatonin was able to inhibit growth/viability and proliferation, to reduce the invasiveness and metastatic properties of human estrogen-sensitive breast adenocarcinoma MCF-7 cell line cultured in growth medium, with substantial impairments of epidermal growth factor (EGF) and Notch-1-mediated signaling. The purpose of this work was to evaluate on MCF-7 cells the possible effects of melatonin on the biophysical features known to have a role in proliferation and differentiation, by using the patch-clamp technique. Our results show that in cells cultured in growth as well as in differentiation medium melatonin caused a hyperpolarization of resting membrane potential paralleled by significant changes of the inward Ca2+ currents (T- and L-type), outward delayed rectifier K+ currents and cell capacitance. All these effects are involved in MCF-7 growth and differentiation. These findings strongly suggest that melatonin, acting as a modulator of different voltage-dependent ion channels, might be considered a new promising tool for specifically disrupting cell viability and differentiation pathways in tumour cells with possible beneficial effects on cancer therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 758, 5 July 2015, Pages 40-52
Journal: European Journal of Pharmacology - Volume 758, 5 July 2015, Pages 40-52
نویسندگان
Roberta Squecco, Alessia Tani, Sandra Zecchi-Orlandini, Lucia Formigli, Fabio Francini,