کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5839545 | 1123994 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Up-regulation of Fas/FasL activation contribute to the apoptosis enhancement of RU486 by Gong-Qing Decoction, a traditional Chinese prescription
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کلمات کلیدی
IODGQDRU486MifepristoneFADDMSDTCMIgGFas/FasL - Fas / FaslFpA - FPAstandard deviation - انحراف معیارimmunoglobulin G - ایمونوگلوبولین GTem - این استintegral optical density - تراکم نوری یکپارچهTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling - ترمینال deoxynucleotidyl transferase-mediated dUTP نام نهایی پایان نامهTUNEL - تونلApoptosis - خزان یاختهایUterine bleeding - خونریزی رحمgestational age - سن حاملگیtraditional Chinese medicine - طب سنتی چینیTransmission electron microscope - میکروسکوپ الکترونی انتقالFas-associated protein with death domain - پروتئین مرتبط با Fas با دامنه مرگcaspase - کسپاز یا کاسپاز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aim of the studyTo elucidate the mechanisms of Gong Qing Decoction(GQD) on human trephocytes and decidual cells in vivo based upon the effective practice of alleviating uterine bleeding in RU486 medical abortion.Materials and methods90 intrauterine pregnancy women within 7 weeks, presenting for elective termination of pregnancy, were divided into the GQD-RU486 group, the RU486 group and the vacuum aspiration group. Duration of uterine bleeding was recorded and volume of uterine bleeding was measured by the method of alkaline hematin photometric. Ultramicrostructure of trephocytes and decidual cells were observed with transmission electron microscope (TEM), and apoptosis rate (AR) was assessed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. At the same time, immunohistochemical staining was performed and integral optical density was analyzed to evaluate the protein expression of Fas, FasL, Caspase-8 and Caspase-3 in both trephocytes and decidual cells preliminarily.ResultsIn comparison with the RU486 group and the vacuum aspiration group, both the duration and volume of uterine bleeding decreased significantly in the GQD-RU486 group. At the same time, both trephocytes and decidual cells in the GQD-RU486 group showed typical character of apoptotic ultramicrostructure and displayed up-regulated apoptosis rate. Synchronously, the integral optical density showed increased protein expression of Fas, FasL, Caspase-8 and Caspase-3 in both trephocytes and decidual cells in the GQD-RU486 group compared with other groups.ConclusionThese data suggest that GQD can alleviate uterine bleeding effectively in RU486 medical abortion by way of apoptosis induction. The apoptosis enhancement of RU486 by GQD may be attributable to the activation of Fas and FasL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 134, Issue 2, 24 March 2011, Pages 386-392
Journal: Journal of Ethnopharmacology - Volume 134, Issue 2, 24 March 2011, Pages 386-392
نویسندگان
Xia Li, Ruifen Liu, Weidong Zhang, Bin Wang, Ruiping Ma, Xuezhi Chi, Li Wang, Xianbin Zhou, Yuqi Guo, Guosheng Jiang, Chengfang Yao,